Jilin Province Great Great Pharmaceutical Co. LTD

Jilin Province Broadwell Pharmaceutical Co., LTD. Welcome you!

Service hotline:+86-431-85639055

博大伟业
imgboxbg

HEALTH SCIENCE

Your current location:
Homepage
/
/
/
Ruilaisheng | With its unique mechanism, can it be better?

Ruilaisheng | With its unique mechanism, can it be better?

  • Categories:Stomach healthy
  • Author:
  • Origin:
  • Time of issue:2020-12-04
  • Views:0

(Summary description)

Ruilaisheng | With its unique mechanism, can it be better?

(Summary description)

  • Categories:Stomach healthy
  • Author:
  • Origin:
  • Time of issue:2020-12-04
  • Views:0
Information

  Zinc preparations can prevent gastric mucosal damage in a variety of animal models, such as reserpine and alcohol-induced gastric mucosal damage, and can also increase the rate of ulcer healing. This anti-ulcer effect is related to maintaining the gastric mucosal barrier and stimulating mucus secretion. Zinc acetaminophen (zinc preparation) can protect rats from gastric mucosal damage caused by alcohol, 25% NaCl and 0.6NHCl. This protective effect has nothing to do with inhibiting gastric acid secretion, but is similar to the cytoprotection of prostaglandins (PGs) effect. dmPGE2 can effectively protect gastric mucosal damage caused by alcohol. L-carnosine accelerates the healing of ulcers by promoting the growth of granulation tissue, but it cannot prevent gastric mucosal damage caused by reserpine or stress.

  Relysun (Polyprezinc particles), a chelate composed of zinc ions and L-carnosine, is a new type of anti-ulcer drug. The design idea is that L-carnosine combined with zinc ions may be caused by zinc ions. Exist and enhance the anti-ulcer effect. This study aimed to explore the effect of polyprezinc on alcohol-induced gastric mucosal and cell damage, and to explore its relationship with endogenous PGE2.

  Materials and Methods

  1.1 Animal

  601 200g Wistar rats were selected. 18h before the experiment, they were placed in a separate cage to prevent autophagy; they were fed in a normal manner before sacrifice.

  1.2 Experimental method

  To explore the protective effect of polyprezinc on alcohol-induced gastric mucosal injury, 160 rats were given intragastric polyprezinc (3, 10 and 30 mg/kg) and normal saline. After 1, 2, 4, and 6 hours, the rats were perfused Stomach 2mL absolute ethanol; 120 rats were given the same dose of polyprezinc and saline, 30min, 1h and 2h, 2mL absolute ethanol was given to the stomach; 80 rats were subcutaneously injected with indomethacin (5mg/kg ) And normal saline. After 30 minutes, the same dose of polyprezinc and normal saline were administered intragastrically. After 1 hour, give 2 mL of absolute ethanol to the stomach.

  To compare the effects of polyprezinc and its separate components zinc ions and L-carnosine, 69 rats were given intragastric polyprezinc (10-30mg/kg), ZnSO4·7H2O (10 and 30mg/kg), L-carnosine (7 and 23mg/kg) and physiological saline, 1h later, gavage 2mL of absolute ethanol. All rats were sacrificed 5 minutes later, and the gastric mucosa was assessed for visible damage.

  To evaluate the degree of gastric mucosal damage, 40 rats were given intragastric polyprezinc (10 and 30mg/kg), dmPGE2 (5μg/kg) and normal saline. After 1 hour, they were given 2mL absolute ethanol. All rats were sacrificed 5 minutes later, and the mucosal injury area (destruction of the epithelial tissue surface and deep necrosis) was measured with an optical microscope.

  To evaluate the effect of L-carnosine zinc on the level of PGE2 in gastric mucosa, 20 rats were given intragastric polyprezinc (10 and 30 mg/kg) and normal saline. One hour later, they were sacrificed and the gastric mucosa was taken to determine the level of PGE2. In the other group, the effect of polyprezinc on PGE2 levels during intraperitoneal administration was studied. Twenty-eight rats were intraperitoneally injected with polyprezinc (10 and 30 mg/kg) and normal saline, and were sacrificed 30 minutes later.

  To evaluate the effect of polyprezinc on gastric acid secretion, 32 rats were given intragastric polyprezinc (3, 10 and 30 mg/kg) and normal saline, and the pylorus was ligated 1h later. Another 32 rats were intraperitoneally injected with the same dose of polyprezinc and normal saline immediately after pyloric ligation. The volume and acidity of stomach contents were measured 4h after ligation.

  1.3 Prostaglandin determination

  PGE2 was measured by radioimmunoassay of rabbit anti-PGE2 bovine serum albumin serum. The radioactivity was counted with a RackBeta 1215 liquid scintillation counter.

  1.4 Evaluation of mucosal damage

  The total length of the lesion is used as the mucosal lesion score.

  1.5 Mucosal histologically coded mucosal specimens were evaluated under light microscope. By measuring the length of the mucosal strips and the total length of the necrotic lesions of each strip, the degree of tissue necrosis with a depth of more than 0.15 mm was estimated from the morphology with the aid of a micrometer. In the same way, we estimate the interruption of the continuity of the surface epithelium by measuring the length of the mucosa without surface epithelium, and express it as a percentage of the total length of each mucosa.

  1.6 Determination of stomach contents

  4 hours after ligation of the pylorus, all stomach contents were collected, and the volume and acidity were measured with an autolysis titrator of a pH meter.

  Statistical Analysis

  The results are expressed as mean ± standard deviation, and the statistical significance of the difference is evaluated by analysis of variance with the subsequent t test. P<0.05 has a significant difference.

  results and analysis

  2.1 The effect of polyprezinc on alcohol-induced gastric mucosal injury in rats

  Polyprezinc has a dose-dependent protective effect on gastric mucosa. The effective doses are 10 and 30 mg/kg, regardless of the route of administration. This protective effect appeared after 1, 2, and 4 hours after the administration of Polyprezinc, and disappeared after 6 hours. When administered intraperitoneally, the protective effect was observed only 30 minutes after the administration and disappeared thereafter.

  Figure 1 The effect of intragastric polyprezinc on alcohol-induced gastric mucosal injury in rats

  2.2 The protective effect of polyprezinc on gastric mucosa is not interfered by indomethacin. Subcutaneous injection of indomethacin (5mg/kg) in advance, administration of polyprezinc (10 and 30mg/kg) has a better effect on the gastric mucosa The protective effect.

  2.3 The effect of polyprezinc, ZnSO4·7H2O and L-carnosine on alcohol-induced gastric mucosal damage

  Compared with the same amount of zinc, the protective effect of polyprezinc is more than three times that of ZnSO4·7H2O, but the same dose of L-carnosine cannot prevent the damage (Table 2), indicating the protection of polyprezinc on the gastric mucosa The effect mainly comes from zinc ions.

  2.4 The effect of polyprezinc and dmPGE2 on alcohol-induced gastric mucosal injury

  In tissue examination, after 5 minutes of intragastric administration, alcohol caused extensive rupture of epithelial cells and deep mucosal necrosis. When polyprezinc (10 and 30mg/kg) and dmPGE2 (5μg/kg) were administered, deep necrosis was inhibited, but both drugs had no inhibitory effect on the rupture of epithelial cells.

  2.5 The effect of polyprezinc on the level of prostaglandin E2 in the gastric mucosa of rats

  Regardless of intragastric administration or intraperitoneal administration, polyprezinc (10 and 30mg/kg) does not affect the level of prostaglandin E2 in the gastric mucosa.

  2.6 The effect of polyprezinc on the secretion of gastric contents in rats with pyloric ligation

  The commonly used dose of polyprezinc by intragastric administration has no obvious effect on the acid secretion and acid output of pyloric ligated rats. However, when the intraperitoneal dose is 10 mg/kg or more than 10 mg/kg, gastric acid secretion is affected. Obviously inhibited.

  2.7 The protective effect of polyprezinc and dmPGE2 on cells in vitro

  In the cell suspension, surface epithelial cells accounted for 81% of the total number of cells, mucous neck cells accounted for 6%, parietal cells accounted for 10% and principal cells accounted for 3%.

  In the trypan blue incompatibility test, cell viability was not affected by 10-4M indomethacin before intragastric administration with 15% alcohol. Cells were damaged within 5 minutes of exposure to 15% alcohol. Polyprezinc and dmPGE2 significantly inhibit cell damage; the protective effect of polyprezinc and dmPGE2 on cells in vitro can still be observed when indomethacin (10-4M) is pretreated.

  "The same dose (10-6M) of polyprezinc and dmPGE2 can also inhibit the reduction of lactate dehydrogenase caused by 15% alcohol.

  Discuss

  Polyprezinc can effectively protect the gastric mucosa, and the protective effect is dose-dependent, and is not interfered by indomethacin; compared with the equivalent amount of zinc, it is known that the protective effect of polyprezinc on gastric mucosa is mainly derived from zinc ions. L-carnosine has a synergistic effect. At the same time, both polyprezinc and dmPGE2 can inhibit deep mucosal necrosis. Whether intragastric or intraperitoneal administration of polyprezinc does not affect the level of mucosal prostaglandin E2, and prevents gastric mucosal damage caused by alcohol. Polyprezinc also has a significant protective effect on gastric mucosal cells in vitro, but indomethacin does not have this effect.

  This shows once again that Boda Weiye Ruilaisheng (Polyprezinc Granules) is a mucosal protective agent with a unique mechanism of action. The perfect combination of zinc ions and L-carnosine synergistically makes it play a better protective effect.

Scan the QR code to read on your phone

Jilin Province Great Great Pharmaceutical Co. LTD

Service hotline

+86-431-81158731

CONTACT US

Jilin Province Great Great Pharmaceutical Co. LTD

Jilin Province Great Great Pharmaceutical Co. LTD
Jilin Province Great Great Pharmaceutical Co. LTD
Jilin Province Great Great Pharmaceutical Co. LTD
Jilin Province Great Great Pharmaceutical Co. LTD
Jilin Province Great Great Pharmaceutical Co. LTD
Jilin Province Great Great Pharmaceutical Co. LTD

Liaoyuan Production Base: No. 158 Fortune Road, Liaoyuan Economic Development Zone, Jilin Province

Tel:+86-437-6998023

Changchun R&D Center: No. 3786 Juye Street, Jingyue Development Zone, Changchun City, Jilin Province
Tel:+86-431-81158731(Marketing center)

Tel:+86-431-81158756(Research and development center)

Beijing Office: Room 901, Building F, Kaixuan City, 170 Beiyuan Road, Chaoyang District, Beijing
Tel:+86-10-58236233

Medication consultation, feedback on medication adverse reaction, and user complaint telephone:0437-5029815 

NO PUBLIC

Jilin Province Great Great Pharmaceutical Co. LTD

Scan and follow the official official account

Copyright © Jilin Province Great Great Pharmaceutical Co. LTD        吉ICP备20002630号

Website construction:300.cn Changchun   management     

Jilin Province Great Great Pharmaceutical Co. LTD