A large number of studies in Japan have confirmed that the inhibitory effect of Polyprezinc on Helicobacter pylori (Hp) stems from the direct inhibitory effect on Hp flora. There is also a study by examining the effect of polyprezinc on Hp mouse gastric mucosal inflammatory lesions, including microcirculation white blood cell activation and subsequent oxidation reactions, and exploring its related mechanisms.

　　Study and compare the changes in the concentration of anti-Helicobacter pylori IgG antibody in serum. The results showed that the IgG concentration of all gerbils in the Hp group and the Hp+polyprezinc group was significantly increased. The serum titer of the Hp combined with polyprezine group was lower than that of the Hp group, but the serum titer of the Hp combined with polyprezinc group was significantly higher In the control group or polyprezinc group. At the same time, the study compared the density of Hp flora in each group, and the results showed that the flora density of the control group and the polyprezine group was zero, and the bacteria in the gastric antral mucosa and gastric mucosa of the gerbils in the Hp combined with polyprezinc group The population density was significantly lower than that of the Hp group; the Hp flora density and myeloperoxidase activity in the Hp group and the Hp combined polyprezinc group were compared, and it was found that the Hp combined polyprezinc group had a significant myeloperoxidase activity Lower than Hp group.

　　At the same time, the study observed the morphology of the gastric mucosa 12 weeks after Hp inoculation. The study found that there was no obvious gastric mucosal damage in the Hp+polyprezinc group. The gerbils in the Hp group showed obvious inflammation in the mucosa and submembrane, accompanied by infiltration of polymorphonuclear and monomorphonuclear cells. However, no obvious inflammatory reaction was found in the Hp+polyprezinc group. The degree of acute inflammation in the Hp group was significantly higher than that in the control group. In the Hp combined with polyprezinc group, the acute inflammatory response was significantly suppressed.

　　At the same time, 12 weeks after inoculation, the gastric mucosa was observed under a biomicroscope, which clearly showed that the number of white blood cells adhering to the mucosal endothelium and submucosal venules increased. However, in the Hp combined with polyprezinc group, the number of white blood cells adhering to the small veins of the gastric mucosa did not increase significantly.

　　In vitro antibacterial study (1)

　　Based on the above-mentioned relevant research data, Boda Weiye conducted a study on the in vitro antibacterial activity of Ruilaisheng in 2017 with the Chinese Center for Disease Control and Prevention (CDC) Institute for Infectious Disease Control and Prevention;

　　Purpose:

　　examine the inhibitory effect of Relaisen, carnosine and other zinc preparations on Hp

　　Experimental materials and methods:

　　Strain source: Hp international standard strain (700392) and domestic isolate (723813). All strains are provided by CDC.

　　Experimental results: Concentration of Relaisen that completely inhibits the growth of Hp:

　　0.04mmol/ml, 0.03mmol/ml, 0.02mmol/ml, 0.01mmol/ml, 0.005mmol/ml, 0.0025mmol/ml

　　In vitro antibacterial study (2)

　　In 2018, a confirmatory study was conducted with the Institute of Geriatrics, General Hospital of the People's Liberation Army;

　　Test purpose: To further verify the inhibitory effect of Relaisen on Hp;

　　Experimental materials and methods:

　　7 clinical strains, Hp international standard strain (11637)

　　Experimental method: agar dilution method

　　test results:

　　clinical strains: 2 strains MIC: 0.001 mmol/ml, 5 strains MIC: 0.0025 mmol/ml;

　　Hp International Standard Strain (11637): MIC: 0.001 mmol/ml Water and HCL as a solvent have the same dissolving ability for the effective antibacterial components of Relaisen, which further indicates that the antibacterial activity of Relaisen is relatively affected by the pH value of the stomach. small.

　　The above-mentioned research results Boda Weiye obtained a national invention patent:

　　Discuss

　　A large number of studies have confirmed that the effect of polyprezinc on Hp can be attributed to zinc ions. At the same time, it is believed that its antibacterial activity in the stomach is significantly better than in vitro, because its chelating structure can effectively adhere to the injured gastric mucosa; at the same time, the pylorus Helicobacter (Hp) bacteria contain a large amount of active urease inside and outside the body, which is an important pathogenic factor of Hp infection. So, how does polyprezinc affect urease activity?

　　Urease has a complex structure, with a thickness of about 13nm and a regular triangle symmetry. It is a nickel metalloenzyme, which is determined by the nickel content of the active site. The urease operon contains 7 genes, namely ureABIEFGH. Among them, ure A and ure B are structural genes, ure EFGH is an accessory gene, and ure I is a gene specific to Hp urease. The urease accessory gene can assist the insertion of nickel into the active site of the UreA/ UreB protein to form an active protein. The activation of the enzyme activity of urease requires the insertion of two nickel in the six active sites of the holoenzyme. Therefore, the protein encoded by the accessory gene is in the metal The polymerization of nickel to the active site of the enzyme plays a very important role. Therefore, polyprezinc mainly affects the activity of urease through three ways:

　　一. Displacement modification of zinc ions: Zinc can cause nickel ions in the urease molecule to undergo a substitution reaction, which changes the conformation of urease, thereby significantly inhibiting its enzyme activity;

　　2. A variety of proteins can regulate the activity of urease by affecting whether metal ions can be inserted into the enzyme structure normally. These protein factors include: NixA, NikR, HspA and HspB, Hpn, among which Hpn is a histamine-rich protein, which can bind nickel or zinc. Another important component of polyprezinc is carnosine and alanine. Two peptide units composed of amino acids, histidine can effectively regulate the accurate insertion of zinc ions into the enzyme structure site, thereby significantly inhibiting the activity of urease.

　　3. Histidine (His) is necessary for the HspA protein to bind zinc ions to cause the inactivation of urease. It can also effectively regulate the accurate insertion of zinc ions into the active site of the zymogen and the substitution reaction with nickel ions. The conformation of this residue changes Make urease lose nearly 98% of its enzyme activity.

　　Boda Weiye Ruilaisheng-Polyprezinc Zinc Granules can accurately attack the important pathogenic factor urease of Hp infection, thereby significantly improving the eradication rate of Hp.