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Gastric mucosal protective agent with dual mechanisms of protection and repair-Polyprezinc particles

Gastric mucosal protective agent with dual mechanisms of protection and repair-Polyprezinc particles

  • Categories:Stomach healthy
  • Author:
  • Origin:
  • Time of issue:2020-12-03
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(Summary description)

Gastric mucosal protective agent with dual mechanisms of protection and repair-Polyprezinc particles

(Summary description)

  • Categories:Stomach healthy
  • Author:
  • Origin:
  • Time of issue:2020-12-03
  • Views:0
Information

  Preface

  As we all know, zinc is known as the flower of intelligence and the element of life in the medical field, which can accelerate the migration of epithelial cells and promote the growth of granulation tissue; while carnosine is composed of L-histidine and β-alanine and is found in mammalian skeletal muscle It has the effect of promoting wound healing and immune regulation. Polyprezinc, a chelate composed of zinc ions and L-carnosine, is an effective drug commonly used clinically to treat gastric ulcers. Studies have shown that polyprezinc protects various types of acute experimental gastric and duodenal injuries in rats. So does polyprezinc benefit from chronic peptic ulcer? We use acetic acid to induce gastric mucosal damage in rats, which is very similar to human chronic peptic ulcers. Explore the protective effect of polyprezinc on gastric mucosal damage At the same time, we used hydrocortisone to induce ulcer recurrence to explore the preventive and repairing effects of polyprezinc on ulcer recurrence.

  Materials and Method

  1.1 Animal

  Use male SD rats, weighing about 180g. Starting 3 days before the induction of ulcers, only eat between 10:00-11:00 a.m. and 6:00-7:00 p.m. every day.

  1.2 Induction of acetic acid ulcer in rats with restricted feeding time and evaluation of the healing effect of the test drug. Injecting 0.05 mL of 20% acetic acid into the serosal layer of the gastric gland in rats induces gastric ulcer. On the first day after the acid injection, the test drug shall be taken orally twice a day ( Polyprezinc, ZnCl2, L-carnosine and gemfarnate, 9:30a.m. and 5:30p.m.; Cimetidine, 11:30a.m. and 7:30p.m.), 0.5 mL/100g body weight for 14 consecutive days. The control group was given vehicle only. The rats were sacrificed on the 15th day, the stomach was taken, and the ulcer index (mm²) was measured; the sections were made and stained with hematoxylin and eosin (HE), and histological observation was performed under an optical microscope.

  Observe the ulcer site: A: mucosal defect; B: mucosal muscle rupture degree; C: marginal mucosa height; BT: ulcer base thickness; DA: ulcer site defect area; R1+R2: regenerated mucosal area. Calculate the index based on the above data: Exposure ulcer base reduction index=[(B-A)/B]×100, mucosal regeneration index=[(R1+R2)/(C×B)]×100. The effectiveness of the drug is evaluated by comparing the above data with the control group.

  1.3 Hydrocortisone induces the recurrence of acetic ulcer in rats with restricted feeding and evaluation of the preventive effect of the experimental drug

  "Induced acetic acid ulcer in rats as described above, and injected 20 mg/kg of hydrocortisone acetate every day from day 40 to day 59 after acid injection to induce ulcer recurrence. During the above-mentioned ulcer healing process, the test drug was taken orally twice a day from day 40 to day 59. The control group was given vehicle only. The rats were sacrificed on the 60th day, and the preventive effect of the test drug on recurrent ulcers was evaluated by histological measurement. Observe the healing of the ulcer, measure the ulcer index, the defect area of ​​the ulcer and the thickness of the ulcer base, and evaluate the efficacy of the test drug. In addition, calculate the ulcer base exposure index=[A/B]×100, and regenerate mucosal index=[(R1+R2)/(B×C)]×100. The calculation formula of the latter index is the same as that of the mucosal regeneration index. In the case of ulcer recurrence, the ulcer base that has been completely covered by the regenerated mucosa is exposed again due to the loss of the regenerated mucosa, so it is called the regenerating mucosal index.

  1.4 Statistical analysis

  The results are expressed as mean±S.E, and the data adopts one-way analysis of variance and Kruskal-Wallis analysis. Student's test and two-tailed Mann-Whitney U test were used to determine the difference between the two groups.

  Results and analysis

  2.1 The healing effect of polyprezinc, gemfaxate and cimetidine on ulcer

  After treatment with polyprezinc (3 and 10 mg/kg), the ulcer index was reduced by 54% and 65%, respectively; cimetidine (100 mg/kg) and gemfaxate (300 mg/kg) treated ulcers The index dropped by 61% and 57%, respectively.

  After treatment with polyprezinc (3 and 10 mg/kg), the defect area of ​​the ulcer site was reduced by 58% and 64%, respectively; after treatment with cimetidine (100 mg/kg) and gemfaxate (300 mg/kg) The ulcer index dropped by 70% and 67%, respectively.

  When the ulcer base is completely covered by the regenerated mucosa, the ulcer base exposure reduction index is designated as 100. Only polyprezinc (10 mg/kg) and cimetidine (100 mg/kg) significantly improved the index, increasing by 57% and 72%, respectively.

  When the ulcer base completely covers the regenerated mucosa and the height of the mucosa at the edge of the ulcer is equal, the mucosal regeneration index is 100. Polyprezinc (3 and 10mg/kg) increased the mucosal regeneration index by 40% and 60%, respectively. Cimetidine (100mg/kg) increased the index by 90% after treatment, but there was no significant difference between the two. Gemfarmate has no significant effect on this index.

  2.2 The healing effect of ZnCl2 and L-carnosine on ulcer

  ZnCl2 and L-carnosine were treated with doses of 4.7 and 7.7 mg/kg (contained in 10 mg polyprezinc), and the results showed that ZnCl2 and L-carnosine were not effective in promoting ulcer healing either alone or in combination. In addition, even at 10 times the dose, ZnCl2 and L-carnosine alone have no effect.

  2.3 Hydrocortisone induces ulcer recurrence

  On the 40th and 60th day after the acetic acid injection, the base of the ulcer of the rat that was not treated with hydrocortisone was completely covered by the regenerating mucosa. On the 60th day, steroid-treated rats were exposed to the base of the ulcer due to the loss of regenerated mucosa, indicating that hydrocortisone pretreatment for 20 days can cause the recurrence of the ulcer.

  2.4 The healing effect of polyprezinc, gemfaxate and cimetidine on ulcer recurrence

  After treatment with polyprezinc (3 and 10 mg/kg), the ulcer index decreased by 64% and 60%, respectively. The ulcer index decreased by about 60% after treatment with cimetidine (100 mg/kg) and gifani (300 mg/kg).

  After treatment with polyprezinc (3 and 10 mg/kg) and Gifani (300 mg/kg), the area of ​​the ulcer site defect was reduced by 62%, 54% and 61% (Figure 6B). Cimetidine (100 mg/kg) treatment only reduced the defect area of ​​the ulcer site by 43%.

  After treatment with polyprezinc (3 and 10 mg/kg) and Gifani (300 mg/kg), the exposure index of the ulcer layer was reduced by about 55% (Figure 6C). However, cimetidine (100 mg/kg) treatment only reduced the ulcer layer exposure index by 36%.

  After treatment with polyprezinc (3 and 10 mg/kg), the regenerated mucosal index increased by 124% and 119%, respectively (Figure 6D). Cimetidine (100 mg/kg) treatment increased the index by 92%. Gifani (300 mg/kg) increased by 157%.

  Discuss

  It can be seen that for acetic acid-induced ulcers, polyprezinc treatment can not only reduce the size and depth of the ulcer, but also promote the regeneration of the mucosal defect. For the recurrence of hydrocortisone-induced ulcers, polyprezinc treatment can It can effectively prevent the regenerated mucosa from falling off. This protective and preventive effect may be partly attributed to the adhesion of polyprezinc to the ulcer site and the PG-independent cell protection and membrane stabilization effect.

  Ruilaisheng (Polyprezinc Granules) is a drug that can promote ulcer healing and prevent ulcer recurrence. It is a gastric mucosal protective agent with dual mechanisms of protection and repair.

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Jilin Province Great Great Pharmaceutical Co. LTD
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