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The targeted adhesion and repair effects of polyprezinc on oral mucositis

The targeted adhesion and repair effects of polyprezinc on oral mucositis

  • Categories:Stomach healthy
  • Author:
  • Origin:
  • Time of issue:2020-12-01
  • Views:0

(Summary description)

The targeted adhesion and repair effects of polyprezinc on oral mucositis

(Summary description)

  • Categories:Stomach healthy
  • Author:
  • Origin:
  • Time of issue:2020-12-01
  • Views:0
Information

 

 Preface

  Anti-cancer drugs, radiation, and bacterial infections can cause stomatitis. Regardless of the severity of the symptoms, they can cause considerable pain and seriously affect the quality of life of patients. At present, clinically commonly used bactericides, free radical production inhibitors, local anesthetics and anti-inflammatory/analgesic drugs are used to prevent and treat stomatitis, but the efficacy is not satisfactory. Many patients have recurrence after the lesion has healed. Polyprezinc (Polaprezinc, PZ) is a chelate composed of zinc ions and L-carnosine, and is widely used clinically to treat gastric ulcers. Polyprezinc mainly protects the gastric mucosa through antioxidant effects, and promotes mucosal healing by promoting the expression of insulin-like growth factor I (IGF-I). Studies have shown that a mixture of polyprezinc and sodium alginate can be used to treat patients with severe stomatitis. This study aims to examine the potential benefit of polyprezinc in the treatment of stomatitis, and to explore the effectiveness of polyprezinc in the treatment of stomatitis.

  1 Materials and methods

  1.1 Animals: 4-week-old male golden Syrian hamsters (about 50g) were used. After adapting to the environment for at least 4 days, each group randomly selected 6-7 animals for experimentation. 1.2 Acetic acid-induced stomatitis The Tsukimi method was used to establish a stomatitis model. Pentobarbital (50 mg/kg, ip) anesthetize the hamster, extend its cheek pouch outside the mouth, clamp the center of the cheek pouch with 6 mm inner diameter tweezers, and use an amicro syringe to place it under the mucus in the area held by the ring tweezers Inject 30µL of 10% acetic acid solution, return the cheek pouch to the mouth, and raise the animal normally. On days 4-14 after induction of stomatitis, the animals were killed with ether. The cheek pouch was removed, fixed with 2% formalin, and the area (mm²) of each lesion was measured under a stereo microscope. From the second day of induction of stomatitis, polyprezinc was used to treat the lesions of the cheek pouch once a day for 3-13 days. The control group was treated with normal saline. 1.3 Histopathology The hamster cheek pouch was removed 7 days after local injection of acetic acid solution and fixed in 10% phosphate buffer-formalin solution. After washing with ethanol and xylene several times, the tissue was filled with paraffin. After the paraffin sections are solidified, they are prepared according to the original method. After staining with hematoxylin and eosin, observe the sections under a microscope. 1.4 Adhesion of polyprezinc to mucosa After local injection of acetic acid solution for 4 days, apply polyprezinc solution (10mg/kg) to the cheek pouch. The animals were sacrificed at 0.5, 1, and 3 hours after administration, the cheek pouch was cut out, washed repeatedly with normal saline, and the zinc adhesion of the cheek pouch mucosa was detected by reaction with 0.5% dithizone solution. 1.5 The results of statistical analysis are expressed as mean ± standard error. Unpaired Student's t test (∝=0.05) was used to test the significance of the differences between the groups. The p value for negating the null hypothesis is 0.01 or 0.001.

  2 Results and analysis

  2.1 Polyprezinc accelerates the healing of oral mucosal injuries

  4 days after local injection of acetic acid solution, obvious round white lesions (36.9±4.2mm²) were observed in the central area of ​​the cheek pouch (Figure 1). The size of the lesion decreases with time (Day 7: 11.8±1.2mm²; Day 10: 6.6±0.5mm²; Day 14: 2.4±0.2mm²). Compared with the natural healing of the control group, the polyprezinc (10mg/kg) treatment group has a tendency to accelerate the wound healing on the 4th day (lesion area: 33.1±3.1mm²), and significantly accelerate the wound healing from the 7th day (the 7th day). 7 days: 6.3±0.4mm², p<0.01; day 10: 2.5±0.5mm², p<0.001; day 14: 1.2±0.2mm², p<0.001), indicating that polyprezinc can significantly accelerate the acetic acid-induced Oral mucosa injury is healed.

  Figure 1 Changes in the lesion area of ​​ulcerated hamsters caused by acetic acid

  2.2 Polyprezinc reduces oral mucosal damage

  As shown in Figure 2, compared with normal mucosa, 7 days after local injection of acetic acid solution, thickening and damage of mucosa were observed in both the polyprezinc treatment group and the control group. However, despite the thickening of the mucosa in the polyprezinc group, the degree of damage caused by acetic acid was less than that of the control group, and regeneration of damaged tissue was observed after the polyprezinc treatment. It shows that polyprezinc can significantly reduce oral mucosal damage and promote the regeneration of damaged tissues.

  Figure 2 Photos of 7 days after buccal pouch mucosal ulcer caused by acetic acid

  a: normal membrane; b: untreated membrane (7 days after acetic acid injection); c: polyprezinc-treated membrane (7 days after acetic acid injection). (H.E. staining, 20X).

  2.3 Polyprezinc has adhesion to oral mucosa injury

  As shown in Figure 3, the zinc and dithizone complexation coloration method was used to detect the polyprezinc adhering to the mucosa. Figure 3-A, no adhesion of zinc to the normal mucosa was observed, Figure 3-B, C, D, 0.5, 1 and 3 hours after treatment with polyprezinc, the adhesion effect of zinc to the damaged mucosa is obvious, indicating Polyprezinc has adhesion to the injured part of the oral mucosa.

  Figure 3 The photo of the ulcer caused by acetic acid and the adhesion of zinc to the cheek pouch mucosa

  A: control group (before treatment); B: after 0.5h; C: after 1h; D: after 3h. Through the complex color reaction of zinc and dithizone, it can be seen that polyprezinc adheres to the film.

  Discuss

  The pathogenesis of stomatitis is diverse. It is especially common after radiotherapy or taking anti-cancer drugs. Free radicals generated by radiation or anti-cancer drugs damage the oral mucosa. This indicates that drugs with antioxidant activity may be effective in the treatment of stomatitis. Relaisen (Polyprezinc Granules) is a chelate composed of zinc ions and L-carnosine, which protects mucosal damage through antioxidant effects. The results of the study show that for oral mucosal damage caused by acetic acid, polyprezinc can target and adhere to the injured site, significantly reducing the degree of mucosal damage, accelerating the healing of damaged mucosa, and promoting the regeneration of damaged tissues. For clinical stomatitis, polyprezinc can inhibit free radical reactions that lead to delayed healing through its antioxidant effect, and it can also achieve a therapeutic effect by promoting healing. Polyprezinc has a curative effect on stomatitis, and can be widely used in stomatitis caused by radiotherapy or long-term use of anticancer drugs, which greatly improves the quality of life of patients.

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