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Basic Research Experiment Report Ⅳ

Basic Research Experiment Report Ⅳ

  • Categories:Stomach healthy
  • Author:
  • Origin:
  • Time of issue:2020-11-18
  • Views:0

(Summary description)

Basic Research Experiment Report Ⅳ

(Summary description)

  • Categories:Stomach healthy
  • Author:
  • Origin:
  • Time of issue:2020-11-18
  • Views:0
Information
实验名称 聚普瑞锌抑制肝纤维化及肝癌的分子机制研究
项目合作单位 浙江大学
研究者 叶俊、杜勤教授
研究时间 2014年12月- 2016年3月
项目类型 基础实验研究
项目动态 已结题

 

  Experiment

  purpose To explore the protective effect of polyprezinc on liver stress injury, the inhibitory effect of polyprezinc on the production of hepatic stellate cells, and clarify its molecular mechanism; at the same time, evaluate the potential inhibitory effect of polyprezinc on liver malignant tumors It provides a basis for further application of polyprezinc in the protective treatment of gastric mucosa in patients with hepatitis and cirrhosis.

  method

  Using cell proliferation and colony formation assay methods, the inhibitory effect of polyprezinc on the growth of hepatic stellate cells (LX-2) and liver cancer tissues (HepG2) was determined. The migration test was used to evaluate the changes in the migration rate of LX-2 cells, and quantitative polymerase chain reaction was performed to detect the expression levels of key markers of fibrosis. Finally, perform gene chip detection for the treatment of rat liver cancer HepG2 cells to evaluate the effect of polyprezinc on HepG2 gene expression profile.

  result

  The proliferation assay showed that polyprezinc can inhibit the proliferation of LX-2 cells, and the migration test confirmed the inhibition of its migration rate. After polyprezinc treatment, the expression levels of fibrosis markers such as collagen I, fibronectin and α-smooth muscle actin were down-regulated. The proliferation activity of HepG2 cells in the treatment group of rat liver cancer cells was significantly reduced, and the gene chip detection results showed that the expression of a series of genes related to cancer metastasis, cytoskeleton organization and proliferation changed significantly.

  Experimental results

  The results of this study show that polyprezinc treatment can inhibit the cell proliferation of hepatocellular carcinoma and reverse liver fibrosis by inhibiting the activation of HSC. Polyprezinc provides a new treatment method for patients with cirrhosis-related gastritis.

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